Chromosome conformation capture of Ameiurus melas - SRA

SRX14027784: Chromosome conformation capture of Ameiurus melas
1 ILLUMINA (Illumina NovaSeq 6000) run: 68.4M spots, 20.5G bases, 6.1Gb downloads

Design: Hi-C data was generated using the Arima-HiC kit (Ref. 510008), according to the manufacturer's protocols using 10l of blood as starting material, the Truseq DNA PCR-Free kit and Truseq DNA UD Indexes (Illumina, ref. 20015962, ref. 20020590) and the KAPA library Amplification kit (Roche, ref. KK2620). Hi-C library was sequenced in paired end 2*150nt mode on Novaseq6000 (Illumina), using half a lane of a SP flow cell (ref. 20027464). Image analyses and base calling were performed using the Illumina NovaSeq Control Software and Real-Time Analysis component (v3.4.4). Demultiplexing was performed using Illumina's conversion software (bcl2fastq v2.20). The quality of the raw data was assessed using FastQC (v0.11.8) from the Babraham Institute and the Illumina software SAV (Sequencing Analysis Viewer). Potential contaminants were investigated with the FastQ Screen (v0.14.0) software from the Babraham Institute (Wingett & Andrews, 2018)

Submitted by: INRAE (Fish Physiology and Genomics laboratory)

Study: Ameiurus melas isolate:M_S1 Genome sequencing and assembly

PRJNA603265 • SRP300856 • All experiments • All runs

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Genome sequence of the black bullhead catfish (Ameiurus melas)

Sample:

SAMN13926790 • SRS7989207 • All experiments • All runs

Organism: Ameiurus melas

Library:

Name: Ameiurus_melas_HiC2

Instrument: Illumina NovaSeq 6000

Strategy: Hi-C